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Synthesis and Functional Analysis of a Structure‐Switching Aptamer with Homogeneous 3′‐ends
Author(s) -
Baltis Ariel,
Morse Daniel Paul
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb47
Subject(s) - aptamer , ribozyme , tobramycin , rna , homogeneous , chemistry , biophysics , nucleic acid structure , antibiotics , biochemistry , biology , microbiology and biotechnology , physics , gentamicin , thermodynamics , gene
A structure‐switching signaling aptamer that could detect the antibiotic tobramycin was previously produced by a novel in vitro selection strategy. To facilitate the analysis of the mechanism by which this RNA detects tobramycin, the aptamer was synthesized in a manner that ensures that the 3′ ends of the RNA are homogeneous. This was done by incorporating a self‐cleaving ribozyme at the 3′ end. The resulting RNA will be subjected to enzyme and lead ion‐catalyzed hydrolysis to confirm that the ends are homogenous and to analyze the nature of the tobramycin‐induced conformational change.