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Effects of Exogenous Thrombin on Cell Recruitment and Collateral Arteriole Development in the Mouse Spinotrapezius
Author(s) -
Bruce Anthony,
Peirce Shayn
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb440
Subject(s) - arteriogenesis , thrombin , arteriole , collateral , ischemia , microbiology and biotechnology , proteases , serine protease , medicine , chemistry , biology , protease , microcirculation , biochemistry , platelet , enzyme , finance , economics
A method to induce native collateral arteries/arterioles as a protective measure against tissue ischemia events remains elusive. The serine protease, thrombin, is a potent mitogen and activates a variety of cell types to produce growth factors and cytokines. Exogenous delivery has been shown to promote collateral formation in a rabbit hind‐limb model of ischemia. Our study set out to examine the effects of exogenously delivered thrombin on capillary collateral formation in the Balb/c spinotrapezius model. We found that delivery of 1 NIH unit thrombin/gram body weight at a single time point immediately following ischemic injury speeds capillary collateral maturation with diameters achieved increased by up to 75% after 7 days in treated muscles. In addition, we documented in vivo effects on the recruitment of various cells types that influence arteriogenesis. Thrombin or the correct combination of downstream factors could have practical applications in treating ischemic injuries and possibly in inducing the growth and/or development of native collaterals.