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A novel POK protein, ZNF499, induces BAX gene expression with apoptosis
Author(s) -
Kim MinYoung,
Lee KyungMi,
Hur ManWook
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb44
Subject(s) - biology , microbiology and biotechnology , activator (genetics) , apoptosis , cell cycle , transcription factor , gene , genetics
The BTB‐POZ domain containing POK proteins regulate a variety of cellular events such as oncogenesis, cell cycle, differentiation, and development. The functions of most POK proteins are mostly uncharacterized. In this study, we isolated a new POK protein, ZNF499 (also called ZBTB45) by screening POK proteins with a potential of regulating the p53 pathway. We hypothesized that ZNF499 may activate genes of the p53 pathway. FACS data showed that ZNF499 induced G2‐M cell cycle arrest and inhibited cell proliferation. Quantitative PCR showed that ZNF499 activates p53 pathway genes such as p53, p21, BAX. Particularly, ZNF499 has an effect on transcription of p53 and BAX. Activation of BAX gene increased by ZNF 499 depends on the p53 which is also activated by ZNF499. Loss of function study of ZNF499 showed that ZNF 499 increases expression of apoptotic genes, p53 and BAX, not PUMA. Overall, our data suggested that ZNF499 is a potent transcription activator of BAX.