Premium
The effect of estrogen and a high salt diet on arteriolar reactivity to increased tissue oxygen in the spinotrapezius muscle of the female SS rat
Author(s) -
Kunert Mary Pat
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb432
Subject(s) - estrogen , constriction , endocrinology , medicine , ovariectomized rat , arteriole , chemistry , oxygen , microcirculation , organic chemistry
Estrogen may protect against salt induced microvascular dysfunction. Female SS (Dahl Salt Sensitive, SS/JrHsdMcwi) were divided into 2 subgroups: ovariectomized with (OVX +E) or without (OVX‐E) estrogen replacement. Three days prior to the acute experiment the diet of half of the rats was changed from low salt (LS, 0.4% NaCl) to high salt (HS, 8.0% NaCl). On the day of the acute experiment a transverse arteriole in the spinotrapezius muscle was selected, allowed to equilibrate with 0% O2 in the superfusion solution, and then changes in arteriolar diameter to 21% O 2 in the superfusion solution before and after the topical application of HET‐0016 (10μM), a selective inhibitor of 20‐HETE production, were measured. Neither estrogen nor HS affected arteriolar constriction to 21% O 2 . HET‐0016 significantly attenuated arteriolar constriction to 21% O 2 in the SS rat with or without estrogen and on a LS diet. However, the inhibition was greater in the SS rat without estrogen. HET‐0016 did not significantly inhibit the oxygen induced arteriolar constriction in the SS rat with or without estrogen and on a HS diet. These results suggest that a HS diet and estrogen reduce the contribution of 20‐HETE to oxygen induced arteriolar constriction in the spinotrapezius muscle. Funded by an internal UW‐M Research Growth Initiative grant.