Premium
MODIFYING CELLULAR PROPERTIES USING ARTIFICIAL APTAMER‐LIPID RECEPTORS
Author(s) -
O'Donoghue Meghan B,
Xiong Xiangling,
Tan Weihong
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb386
Subject(s) - aptamer , receptor , streptavidin , cell surface receptor , thrombin , cell , chemistry , cell membrane , biotinylation , microbiology and biotechnology , membrane protein , membrane , biophysics , biochemistry , platelet , biology , biotin , immunology
Proteins found on a cell's surface are crucial for cell recognition and signaling. These events are often precipitated by capture of proteins onto the cell surface by membrane receptors. Here we present a quick, one‐step method to add artificial receptors onto cell membranes. Aptamer‐lipid conjugates acting as artificial receptors quickly integrate into the cell membrane, allowing the cell to capture the aptamer's target protein on the cell membrane. Using streptavidin‐aptamer artificial receptors, fluorescently labelled streptavidin was reversibly captured on cell membranes. In addition thrombin modified cells, capable of clotting a buffer containing the thrombin substrate fibrinogen were made by functionalizing the live cells with thrombin‐aptamer artificial receptors. This technology can be translated to modifying a cell with any protein that has an aptamer.