Premium
Characterization of FGF receptors expression on human neutrophils and their contribution to chemotaxis
Author(s) -
Haddad Lydia E.,
Khzam Lara Bou,
Hajjar Fadi,
Merhi Yahye,
Sirois Martin G
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb343
Subject(s) - matrigel , fibroblast growth factor , chemotaxis , fibroblast growth factor receptor , receptor , microbiology and biotechnology , in vitro , biology , immunology , chemistry , biochemistry
Fibroblast Growth Factors (FGFs) can induce inflammatory mediators release by endothelial cells (ECs) and adhesion molecules expression at their surface, thereby favoring neutrophil recruitment and transvascular migration. Neither the expression nor the biological activities that could be mediated by FGFRs have been investigated on human neutrophils. We observed that circulating human neutrophils from healthy individuals expressed varying levels of FGFRs in their cytosol and at their surface. FGFR‐2 was identified as the sole cell‐surface receptor, with FGFR‐1 and ‐4 localizing in the cytosol and FGFR‐3 being undetectable. Using the murine matrigel plug assay, we observed an inflammatory response in FGF‐2‐containing matrigel plugs (inflammatory score ‐ IS: 2.2/3) as compared to control plugs (IS: 0.2/3). In vitro , FGF‐2 stimulation produced a maximal increase of neutrophil migration at 10 −9 M, and by 2.3‐fold as compared to control. Treatment with blocking anti‐FGFR‐2 antibodies abrogated the effect of FGF‐2, while the blockade of FGFR‐1 and FGFR‐4 reduced neutrophil transmigration by 28.9 ± 20.6% and 14.6 ± 13.6%, respectively. In summary, our study is the first one to report FGFRs expression on human neutrophils, with FGF‐2 promoting neutrophil chemotaxis through FGFR‐2 activation. This work was supported by the Canadian Institutes of Health Research and the Heart and Stroke Foundation of Quebec.