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PROTEOMIC PROFILES OF DESMOID TUMORS AND OSTEOSARCOMA OF BONE AND SOMATIC SOFT TISSUE
Author(s) -
RAO UMA N,
CONRADS TOM P,
HOOD BRIAN
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb320
Subject(s) - osteosarcoma , pathology , soft tissue , clusterin , immunohistochemistry , medicine , cancer research , biology , apoptosis , biochemistry
DESMOID TUMORS AND HIGH GRADE OSTEOSARCOMAS OF BONE AND SOMATIC SOFT TISSUE HAVE DISTINCT PROTEOMIC PROFILES Proteomic analysis of recurrent but non metastasizing desmoid tumors, and osteosarcoma (OS) with known metastatic potential occurring in older individuals was performed to identify potential predictive marker proteins for drug resistance and prognosis. Archived samples from nineteen desmoid tumors and fourteen primary osteosarcoma occurring in soft tissue, bone were collected following IRB approval. Five micron thick sections were obtained from paraffin blocks of tumor and scraped, digested and analyzed by mass spectrometry. Protein abundances determined by spectral counting and used for hierarchical cluster analysis. Increase in HSP70, clusterin, emelin, creatine kinase B and carbonic anhydrase noted predominantly in OS. Results were validated for myoglobin and clusterin by immunohistochemical stains.. Proteins at higher abundance in OS relate to oxidative stress and hypoxia. HSP90 and clusterin may be markers of aggressive extraosseous osteosarcoma and may be indicative of drug resistance. The absence of these proteins in desmoids support their being considered benign locally recurrent tumors.