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Microsatellite Instability in Rhesus Colon Carcinoma
Author(s) -
McArthur Mark Jay,
Barnhart Kirstin,
Baze Wallace,
Bernacky Bruce,
Hanley Patrick,
Benavides Fernando,
Perez Carlos
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb319
Subject(s) - microsatellite instability , mlh1 , biology , msh2 , dna mismatch repair , msh6 , microsatellite , lynch syndrome , genetics , colorectal cancer , cancer , gene , allele
Twenty‐five colon carcinoma cases from a specific pathogen free (SPF) breeding colony of Indian origin rhesus macaques ( Macaca mulatta ) were examined and evaluated for pathogenesis. Although, no specific mode of inheritance was identified there appeared to be a genetic bias, as several parent‐offspring or sibling‐sibling pairs were identified. In a subset of cases, DNA from the tumors and normal tissues from the same animal and tested for microsatellite instability (MSI) utilizing a panel of rhesus dinucleotides (MML3S7, MML4S1, MML11S1, MML16S3, MML17S1, MML17S2 and MML18S3) and a human mononucleotide (BAT‐25). All tumors tested exhibited some degree of microsatellite instability. Immunohistochemistical staining for mutL homolog 1 (MLH1), mutS homolog 2 (MSH2) and mutS homolog 6 (MSH6) was performed with loss of MLH1 staining being observed in several tumors. Rhesus colon carcinoma in the Bastrop colony with its early onset disease, microsatellite instability and loss of MLH1 may represent model of Lynch syndrome.