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Evaluation of cytological alterations and genetic polymorphism in clinically normal oral mucosa of smokers
Author(s) -
Bayoumi Fatehia Ali
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb312
Subject(s) - oral mucosa , carcinogen , physiology , allele , medicine , biology , population , gene , pathology , genetics , environmental health
Background Many enzymes involved in detoxification of chemical carcinogen metabolism are polymorphically expressed, with the alleles presenting different enzymatic activities and some of them are associated with cancer susceptibility. Genetic polymorphisms may contribute to our understanding of inter‐individual variation in susceptibility to environmental carcinogens and development of neoplasias. Deletion of GSTM1 and GSTT1genes will lead to complete loss of enzymatic activities. Aim to study the relationship between GSTM1 and GSTT1 genes deletion and the rate of cellular proliferation in oral mucosa of smokers among a sample population of United Arab Emirates. Material and Methods This cross sectional study consisted of 106 non‐smokers and smokers between the age of30–60. Blood samples and oral smears were collected for DNA extraction and cytologic examination of the silver stained NORs (AgNORs).GSTM1 and GSTT1Gene deletions were analyzed using PCR‐based method. Analysis of data was done by using SPSS program. Results There was significant increased number of Agnors in the cells of oral mucosa of smokers if compared with those of non‐smokers and positive correlation between the number of Agnors in smokers and the age (0.81),Pack‐year(0.93),GSTT1 deletion (0.63) and GSTM1 deletion (0.48).Smokers with deletion of GSTT1 and GSTM1 were at a significant high risk of increased cellular proliferation (OR=0.029, 95%CI= 0.006–0.152)for GSTT1 and 0.060 (0.007–0.504) for GSTM1.There was highly significant difference among the group with deletion when compared to the group of no deletion in both genes(p< 0.001). Conclusion There is an association between GSTT1 & GSTM1 deletion and the risk of increased cellular proliferation of the oral mucosa of smokers. Source of Fund: the Medical Research Fund, Dubai Medical College (MRF‐DMCG).