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Production of novel platycodin D derivatives by Aspergilli
Author(s) -
Ahn Hyung Jin,
You Hyun Ju,
Suh JooWon,
Ji Geun Eog
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb308
Subject(s) - chemistry , xylose , sugar , sapogenin , arabinose , food science , biochemistry , fermentation , medicine , alternative medicine , pathology
Platycodin D (PD) is the major saponin components abundant in the root of Platycodon grandiflorum , which has been reported to have anti‐inflammatory and anti‐tumor activities. PD contains sugar residues including glucose unit at C‐3 and apiose‐xylose‐rhamnose‐arabinose residue at C‐28. In the present study, 8 noble PD derivatives with diverse sugar chains were produced by the crude enzyme extracts of Aspergillus niger and A. usamii grown in the presence of various carbon sources. Commercially available enzymes such as hesperidinase, β‐glucosidase, and β‐xylosidase were not effective in cleaving the glycosidic side chains of PD. Depending on the sugar side chains the PD derivatives demonstrated different degree of toxicity against a normal fetal colon cell line. These findings could be informative to obtain novel PD derivatives with reduced cytotoxicity by Aspergilli. This work was supported by Industrialization Support Program for Bio‐technology of Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries (810007‐03‐1‐WT211), Republic of Korea.