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Obesity increases carcinogenesis in a mouse model of basal‐like breast cancer (BLBC)
Author(s) -
Sundaram Sneha,
Freemerman Alex,
Sampey Brante,
Darr David,
Bendt Katharine,
Hua Kunjie,
Troester Melissa,
Makowski Liza
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb304
Subject(s) - medicine , obesity , endocrinology , breast cancer , basal (medicine) , cancer , fat mass , lactation , carcinogenesis , physiology , pregnancy , diabetes mellitus , biology , genetics
To determine whether diet‐induced obesity promotes post‐pregnancy carcinogenesis in a mouse model of BLBC. In this pilot study non‐parous C3‐Tag mice that are genetically prone to develop only BLBC were used. At 10 weeks old, baseline fat and lean mass were estimated using MRI imaging followed by initiation of diets. Mice, randomized into 3 groups, received low fat (10%; control) or high fat (45% or 60%) diets. Caloric intake was measured by determining the gram food intake/mouse/day. Mice were monitored for palpable tumors thrice weekly from the start of diet. Progression of diabetes and obesity was assessed every 2 weeks. Percent changes in fat mass were assessed monthly with MRI. Mice on all three diets did not exhibit any significant changes in blood glucose levels. Caloric intake was significantly higher on the 60% diet compared to the 10% and 45% diets. High fat diet (45% and 60%) fed mice exhibited higher fat mass, compared to mice on 10% diet after 3 months on diet. Diets did not alter tumor burden (tumor number) versus control diet. C3‐Tag mice exhibit an average tumor latency of approximately 18.5 weeks (n=469). Although, there were no differences observed in the 10% and 60% groups from the expected tumor onset, the 45% fat diet group exhibited a significant decrease in tumor onset by 2 weeks. Further analysis of potential diet‐induced alterations in tumor mass using 3‐D ultrasound is ongoing. In sum, an elevation in caloric intake and fat mass with increases in fat content of the diets is evident with no diabetic complications. We conclude that the 45% diet is sufficient to promote obesity and may decrease tumor latency in C3‐Tag mice. (U01 ES019472 NIEHS).