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In vitro fermentation characteristics of select non‐digestible oligosaccharides using infant fecal inocula
Author(s) -
Boler Brittany Vester,
Rossoni Serao Mariana C.,
Faber Trevor A.,
Chow JoMay,
Fahey George C.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb202
Subject(s) - propionate , butyrate , inulin , chemistry , fermentation , prebiotic , feces , zoology , food science , biochemistry , biology , microbiology and biotechnology
Galactooligosaccharides (GOS), gum arabic (GA), HP inulin (HP), 2′‐fucosyllactose (2FL), 6′‐sialyllactose (6SL), and lacto‐N‐neotetraose (LNnT) were incubated (37°C) in semi‐defined medium with fecal inocula from healthy breast‐fed (n=4) or formula‐fed (n=4) infants, and samples collected over 12 h. LNnT, 2FL, and GOS led to rapid drops in pH (P<0.01; 3, 6, and 12 h), with intermediate changes for HP and 6SL, and no change for GA. GOS led to higher acetate production than 2FL at 3 h (P=0.02), and both 2FL and LNnT at 6 h (P<0.05). At 12 h, GOS led to greater production of acetate than the other substrates (GOS>LNnT=2FL>6SL>HP=GA; P<0.05). Other substrates produced more propionate than GA, but only detected at 12 h (6SL>LNnT=GOS=2FL=HP>GA; P<0.05). Butyrate increased to the same extent (P<0.01) for all substrates except for GA. More lactate accumulated for GOS, 2FL, and LNnT compared to HP, 6SL, and GA (P<0.01). GOS led to greater (P<0.01) lactate production compared to HP, 6SL, and GA at 3 h with this trend continuing through 12 h. This study suggests that the human milk oligosaccharides LNnT, 2FL, and 6SL are highly fermentable by infants. This research was supported by Abbott Nutrition.