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Reactive oxygen species‐mediated activation of eosinophils induced by Anisakis simplex
Author(s) -
Sim Seobo,
Park Solah,
Oh Jungmin
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb138
Subject(s) - anisakis simplex , superoxide , eosinophil , degranulation , reactive oxygen species , nadph oxidase , mapk/erk pathway , chemistry , p38 mitogen activated protein kinases , biology , microbiology and biotechnology , biochemistry , immunology , phosphorylation , enzyme , larva , asthma , botany , receptor
Anisakiasis is parasitic disease caused by accidental ingestion of third stage larvae of Anisakis simplex carried by marine fishes or cephalopods. After ingestion, larvae can penetrate the stomach or intestinal wall and cause eosinophilic infiltration, inflammation, and allergic reactions. Although eosinophils are the principal effector cells of nematodes infection and allergic inflammation, the effect of A. simplex larvae on activation of human eosinophils remains largely unknown. Crude extract of A. simplex larvae caused phosphorylation of p38 MAPK and superoxide anion production, and increased surface expression of CD11b and CD69 on eosinophils. Pretreatment of eosinophils with p38 MAPK inhibitor SB202190 or NADPH oxidase inhibitor diphenyleneiodonium chloride reduced superoxide anion production and up‐regulation of CD11b and CD69 on eosinophils induced by A. simplex . However, pretreatment of eosinophils with diphenyleneiodonium chloride did not affect phosphorylation of p38 MAPK. A. simplex induced degranulation of human eosinophils and pretreatment of eosinophils with DPI blocked it. These results suggest that p38 MAPK‐mediated ROS production is required for the Anisakis ‐induced activation of eosinophils.