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Studies on the function of Ufd3/Doa1 in the distribution of sterol in yeast
Author(s) -
Li Guangtao,
Zhao Gang,
Lennarz William J
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb118
Subject(s) - ubiquitin , yeast , microbiology and biotechnology , ergosterol , sterol , ubiquitin ligase , lysosome , biology , chemistry , function (biology) , biochemistry , enzyme , gene , cholesterol
Ufd3/Doa1 (mamallian homologue PLAA) was initially identified as a component involved in the UFD (Ubiquitin Fusion Degradation) pathway. The defects in Ufd3/Doa1 null strain were mainly caused by the loss of ubiquitin levels, because overexpression of ubiquitin can rescue the defects. However, it is still not clear how Ufd3/Doa1maintains the ubiquitin. In addition to the effect on the ubiquitin level, Ufd3/Doa1 was also plays critical roles in sorting of ubiquitinated protein into the vascuolar/lysosome lumen via the MVB pathway. Recently we found that the C‐terminus of PLAA not only interacts with reported p97/Cdc48, but OBP9L, an oxylsterol binding protein like protein. We also confirmed that the interaction between the yeast Ufd3/Doa1 and OSH proteins. Furthermore, we found that yeast Ufd3/Doa1 null strain is sensitive to a polyene antifungal, amphitericin B, which associates with the main component of yeast cell membrane, ergosterol. All these results indicate that Ufd3/Doa1 might play important roles on the sterol system.