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Identification of host cell factors involved in nuclear egress of HSV‐1
Author(s) -
Olarte María Jesús,
Schlieker Christian
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.lb109
Subject(s) - capsid , biology , microbiology and biotechnology , herpes simplex virus , nuclear transport , identification (biology) , viral envelope , nuclear protein , nuclear lamina , nuclear pore , cell nucleus , nucleus , virology , computational biology , gene , virus , genetics , botany , transcription factor
All known members of the herpesvirus family assemble their capsids in the nucleus prior to envelopment in the cytosol. Capsid transport across the nuclear envelope is therefore an essential step in their life cycle. The viral gene products required for this process have been delineated, but little is known about host cell factors involved in this unusual transport route, which is distinct from the classical nuclear transport pathway. In a proteomics experiment aimed at the identification of host cell proteins involved in nuclear egress, we identified Sun2/Unc84B, a constituent of the multimeric LINC complex ( li nker of the n ucleoskeleton and c ytoskeleton) that traverses the nuclear envelope. We demonstrate co‐localization of UL34 and Sun2 to vesicular structures in the perinuclear region, and the relocalization of Sun2 in response to herpes simplex virus‐1 (HSV‐1) infection. These observations lead us to propose that HSV‐1 hijacks Sun2 to drive capsid transport across the nuclear envelope via associated cytoskeletal motors. Funding: Charles Hood Foundation, Ellison Medical Foundation.