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ApolipoproteinA1 promote resolution of lung inflammation and fibrosis in experimental silicosis
Author(s) -
park sung woo,
Lee Eunhee,
lee eunjoo,
Park choon sik
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.999.2
Subject(s) - silicosis , medicine , tunel assay , inflammation , fibrosis , inhalation , lung , apoptosis , pathology , immunology , chemistry , biochemistry , anesthesia , immunohistochemistry
Background Inhalation of silica particles induces silicosis, inflammatory lung disease and eventually generating multiple fibrotic silicotic nodules. Silica is often used as experimental lung fibrosis model. Objectives To evaluate Intranasal treatment of Apolipoprotein A1(ApoA1) have prevent and/or resolving effect on the developing experimental lung silicosis. Methods BALB/C mice were assigned into four groups. Silica suspension(SiO2; 20mg/50ml in saline) is intratracheally instilled at Day 0. ApoA1 was treated at Day 7,8,9 (APOA1_P group) to investigate its prevention effect of silicosis. ApoA1 was treated at Day 16, 17, 18(APOA1_R group) to evaluate its therapeutic effect. The Mice were sacrificed at day 30. Histology, collagen assay and cytokine measurement including TGF‐b1 were performed. Apoptosis were analyzed using TUNEL assay. Measurement and Results Both ApoA1_P and ApoA1_R group have significant reduce the silica‐induced increased in number of inflammatory cells, silicotic nodule fractions and collagen deposition in sham‐ treated mice. TGF‐b1 level in BALF is significantly decreased in both ApoA1 treated group compare to Silica group. TUNEL assay shows that silica induced increased apoptotic cell numbers are significantly reduced in ApoA1 treated lung. Conclusions Local treatment with Apo A1 have preventive and pro‐resolving effect on the experimental lung silicosis.