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Initial evidence for increased glutamine turnover in obese adolescents
Author(s) -
Lenders Carine M
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.983.23
Subject(s) - glutamine , medicine , endocrinology , leucine , percentile , metabolism , chemistry , amino acid , biochemistry , statistics , mathematics
Objectives To examine the relationships of plasma glutamine‐to‐glutamate ratio (Gln/Glu) to markers of energy metabolism and body fat. Methods 58 obese 13–17.9 years olds, screened for a Weight‐Loss Trial (38 females, 8 black, 16 Hispanic). We calculated FM using DXA‐scan and VAT using CT‐scan. Blood tests included amino‐acids (AA), 25(OH)D, and PTH. HOMA_IR calculated based on fasting glucose and insulin levels. Results Median [10–90th percentile] plasma levels included glutamine 524[432;595], glutamate 56[26;98], their ratio 10[5;22], leucine 21[102;151], and BCAA 428[354;505] μmol/L. After adjusting for age, sex, race/ethnicity, Tanner stage, 25(OH)D deficiency, and PTH, Gln/Glu was inversely associated with HOMA_IR (−0.13±0.06 units, p=0.004). After adjustment for covariates including HOMA‐IR, Gln/glu was inversely associated with VAT (−0.2±0.1%), FM (−628±218g) and BMI (−0.3±0.12kg/m 2 ), p<0.05; not lean mass (p>0.05). Discussion While leucine, BCAA, and glutamine levels were similar to Lepage data (1997), glutamate was more than twice as high. Thus, Gln/Glu was half that of healthy adolescents and inversely related to energy metabolism and body fat measures. These results provide initial evidence of increased glutamine turnover in obese adolescents. K23DK082732‐01.