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Dietary glutamate reduced body fat but increased lean body mass in weaning rats
Author(s) -
Sakai Ryosei,
Kuwahara Tomomi,
Kawamata Yasuko,
Nakamura Hidehiro,
Torii Kunio,
Chin Keigi
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.983.12
Subject(s) - lean body mass , endocrinology , medicine , glutamine , weaning , chemistry , triglyceride , body weight , body water , composition (language) , zoology , amino acid , food science , biology , biochemistry , cholesterol , linguistics , philosophy
Although previous studies have suggested a role of dietary glutamate (Glu) for maintenance of normal growth of rats based on the observation of body weight changes, few studies assessed body composition. This study investigated the effects of Glu ingestion on fat and lean body mass in weaning rats. Male SD rats aged at 3wk old were fed fixed amount of diets containing either balanced amino acid (AA) mixture including 4% Glu (control), its Glu‐free mixture (Glu‐free) or low AA mixture (isonitrogenous to the Glu‐free) for 3 weeks. In another experiment, the rats were fed control AA diet, Glu‐free diet with or without additional glycine (Gly) or glutamine (Gln) for 3 weeks. Food intake, body weight and hepatic triglyceride level were measured. Fat and lean body mass were estimated from total body water measured by D 2 O dilution method. Feeding of Glu‐free diet or low AA diet decreased lean body mass but increased both body fat mass and hepatic triglyceride content compared to those of control. Supplementation of Gly and Gln to Glu‐free diet restored body weight gain to that of control, but did not decreased body fat to control level. These results suggest that Glu in general foods is an important nutrient for body growth and maintenance of adequate body composition.