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Antioxidative Effects of Dehydroglyasperin C Isolated from Licorice
Author(s) -
Kim JongSang,
Seo Jiyeon,
Han Jung Hwa,
Hong YeSeul,
Lim JiSun,
Nam Dae Hwan,
Han Min Young,
Jung Chaelim,
Kim Hyo Jung,
Lim Soon Sung,
Lee Choong Hwan,
Park Jung Han Yoon
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.981.16
Subject(s) - antioxidant , chemistry , lipid peroxidation , dpph , abts , nad+ kinase , ex vivo , biochemistry , in vivo , in vitro , pharmacology , enzyme , biology , microbiology and biotechnology
Our previous study showed that dehydroglyasperin C (DGC) isolated from licorice induced phase 2 detoxifying/antioxidant enzymes such as NAD(P)H:quinone oxidoreductase (QR) in mouse hepatoma cells. This study has been conducted to estimate antioxidant effects of DGC by assessing 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical scavenging activity assay, ferric reducing antioxidant (FRA) assay, total phenolic contents, 2,20‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) scavenging activity assay of the compound. We also measured the intracellular ROS inhibiting activity of DGC by performing 2¡7¡‐dichlorfluorescin diacetate(DCFDA) assay in Hepa1c1c7, BPRc1, HCT‐116, and HT‐29 cells, and the inhibitory effects on lipid peroxidation in liver and brain tissues of rats. The experimental results confirmed that DGC acted as a strong antioxidant both in vitro and ex vivo systems.