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Effect of a novel Omegacoeur®/Doluperine® nutritional combination on human embryonic kidney cell viability
Author(s) -
Sottejeau Yoann,
Patel Amit M,
Gerber Ghislaine,
Pierre Sandrine V,
Maixent JeanMichel
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.981.14
Subject(s) - viability assay , hek 293 cells , curcumin , embryonic stem cell , traditional medicine , pharmacology , cell culture , biology , chemistry , biochemistry , cell , medicine , gene , genetics
The nutritional supplements Omegacoeur® and Doluperine® are based on two ancient and unique dietary health traditions. Omegacoeur® is formulated to supply key active components of the Mediterranean diet (omega 3,6,9 fatty acids, garlic, and basil) and the formulation of Doluperine® is based on the Ayurvedic tradition (curcuma, pepper, ginger extracts). Recent studies suggest that a combination of the ingredients supplied by these two supplements could provide additional and previously unanticipated benefit through synergistic actions of some of their key components, but the effect of the combination on human cell viability isn't known. This was investigated in Human Embryonic Kidney (HEK 293) cells. Incremental doses of 4 Omegacoeur®/Dolupérine® combinations prepared so that the molar ratio DHA (Docosahexaenoic acid) in Omegacoeur®/curcumin in Dolupérine® was kept constant, at 2.5 DHA/1 curcumin, were added to the culture media. After 24h of incubation, cell viability was assessed by trypan blue exclusion. The data suggest that combination of Omegacoeur® with Dolupérine® doesn't affect HEK 293 cell viability in the range of doses that have demonstrated beneficial effects in earlier studies. Supported by the French National Agency for Technological Research (ANRT) CIFRE N° 924/2008.