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Antioxidant and Immuno‐stimulating Activity of Sesquiterpene Compounds Isolated from Artemisia iwayomogi in HepG2 and RAW 264.7 Cells
Author(s) -
Jang HaeDong,
Lee SangHyun,
Kim JinKyoung,
Ra JeongChan,
Kim YoungHo
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.981.12
Subject(s) - sesquiterpene , antioxidant , oxidative stress , nitric oxide , chemistry , artemisia , herb , biochemistry , in vitro , traditional medicine , pharmacology , biology , medicine , stereochemistry , medicinal herbs , organic chemistry
Artemisia iwayomogi has been used as a medical herb to prevent liver diseases such as jaundice, acute hepatitis and cirrhosis in Asian countries. In this study, we investigated the antioxidant and anti‐inflammatory activity of sesquiterpene compounds isolated from Artemisia iwayomogi by in vitro and cellular models. The antioxidant activity was estimated using in vitro ORAC and AAPH‐ or Cu2+‐induced HepG2 cell model. The antioxidant activity of sesquiterpene compounds dose‐dependently increased between 1 and 10 uM in ORAC assay system. In AAPH‐ or Cu2+‐induced HepG2 cells, 10 uM of sesquiterpene compounds efficiently reduced oxidative stress when compared to control. The immune‐stimulating activity of sesquiterpene compounds was estimated using DAF‐FM fluorescence probe and western blotting. The increased formation of nitric oxide (NO) and expression of inducible nitric oxide synthase (iNOS) by sesquiterpene compounds treatment may contribute to immune‐stimulating activity of RAW 264.7 cells. These results indicate that sesquiterpene compounds isolated from Artemisia iwayomogi have the potential as a pharmaceutical food to prevent oxidative stress‐related chronic diseases. Grant Funding Source : ASN