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Volatile Components of Mustard Seed induce apoptosis through both caspase‐dependent and ‐independent pathways in human colon cancer cells
Author(s) -
Kim Jaeyong,
Hong Hyeran,
Lee Juhye,
Jeong Ilyun,
Shim Kihwan,
Seo Kwonil
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.979.8
Subject(s) - apoptosis , caspase , dna fragmentation , poly adp ribose polymerase , microbiology and biotechnology , chemistry , caspase 3 , caspase 9 , mitochondrion , fragmentation (computing) , programmed cell death , biology , biochemistry , dna , polymerase , ecology
Mustard seed is used widely as a culinary spice because of the taste associated with the isothiocyanates which are responsible for several beneficial health effects. To investigate the anticancer activity of mustard seed in HT‐29 colon cancer cells, the volatile components including 3 types of isothiocynate from mustard seed were collected by simultaneous distillation extraction method. The volatile components induced apoptotic cell death significantly, resulting in a morphological change, apoptotic DNA fragmentation, and an increase in the sub‐G1 apoptotic cell population. The components triggered the mitochondrial caspase‐dependent apoptotic pathway as shown by an increase in Bax/Bcl‐2 ratios, activation of caspase‐9 and caspase‐3, and cleavage of PARP. In addition, the volatile components also induced AIF release from mitochondria, indicating a stimulated apoptosis through caspase‐independent pathway. These results demonstrated that the volatile components from mustard seed inhibited proliferation of HT‐29 cells through inducing apoptosis, which may be mediated via both caspase‐dependent and ‐independent apoptotic pathways.