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White button, shiitake, and portabella mushrooms inhibit the secretion of vascular endothelial growth factor (VEGF) and the proliferation of the androgen dependent LNCap prostate cancer cell line
Author(s) -
Kuvibidila Solo,
French Christine
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.979.11
Subject(s) - lncap , vascular endothelial growth factor , prostate cancer , cytokine , secretion , cancer cell , cytotoxic t cell , viability assay , cancer research , cell culture , cell growth , endocrinology , angiogenesis , biology , chemistry , medicine , cancer , immunology , cell , vegf receptors , in vitro , biochemistry , genetics
VEGF, a potent angiogenic factor secreted by various normal and cancer cells, has been implicated in cancer growth and metastasis. Plasma and urinary levels of VEGF usually correlate with cancer severity implying that its reduction by bioactive compounds in foods may modulate tumor growth. The antitumor properties of exotic mushrooms have long been known and have been attributed to immunomodulatory, cytotoxic, and cytostatic properties. However, their effect on VEGF is unknown. The goal of this study was to determine whether mushroom extracts inhibit cancer cell proliferation via reduction of VEGF secretion. LNCap prostate cancer cells, 1.25 × 10 5 were incubated with 0–100 μg/ml of ethanol extracts of white button mushrooms (WBM), portabella (PM), and shiitake (SM) for 3, 6, and 24 h in 10% FCS‐supplemented medium. VGEF secreted in the culture medium was assayed by ELISA. Mushrooms extracts had no effect on VEGF secretion after 3 h of incubation, but decreased the levels in a dose dependent fashion after 6 and 24 h (i.e. 100 μg/ml & 24 h: WBM, 19%, PM 46%; SM 43%, p<0.05, n=4 assays). Cell viability and proliferation assessed by MTT test decreased by 5–37%. Reduction of VEGF was not due to a defect in IL‐6 and IL‐8, inducers of VEGF, since neither cytokine was detectable in the same cultures. Although the mechanism has yet to be investigated, our data suggest that edible mushrooms may reduce the risk of metastatis by inhibiting VEGF. Source of support OCAST, Startup funds from Oklahoma State University.