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Ambient fecal reactive oxidative species (ROS) buffering capacity as a function of geography and habitual diet: A comparison of rural and urban Guatemalan subjects
Author(s) -
Orozco Monica Ninnette,
Arriaga Claudia,
Soto Maria Jose,
Hernandez Liza,
Campos Raquel,
Solomons Noel W.,
Schümann Klaus
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.978.1
Subject(s) - feces , reactive oxygen species , oxidative stress , chemistry , zoology , food science , medicine , biology , biochemistry , ecology
Background In the ambient state, fecal matrix has a powerful capacity to buffer ROS induction in an in situ assay system. Objectives To examine factors influencing the buffering capacity of stools across differing dietary habits, with emphasis on dietary fiber (DF), and between sexes. Methods Ambient stool samples were collected on 2 nonconsecutive days in 20 rural women (RW) and 20 urban women (UW), with concurrent 24‐h recalls, and on 3 consecutive days in 27 urban men (UM). An assay for Fe‐induced adducts of salicylic acid in mg/mL units gauged fecal buffering. Stool non‐heme Fe was measured by a colorimetric test. >Results RW consumed 2.5 X the DF as UW: 40±21 vs 16±8 g, p<0.001, but ROS generation was identical across areas: 0.077±0.015 vs 0.078±0.019 mg/mL, p=0.86. UM had ROS levels 150% higher than women: 0.20±0.11 mg/mL, p<0.0001. Fecal non‐heme Fe associated poorly with ROS production, r=−0.05 (p=0.71), and failed to explain sex difference in buffering capacity. >Conclusion High and low DF intakes have no influence on ROS buffering in women, but sex strongly determines ambient oxidative quenching in stools. The latter could explain gender disparities for certain intestinal diseases. Funded by Hildegard Grunow Foundation