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Fish oil modulates cytolytic lymphocytes during H. hepaticus ‐induced colitis in mice
Author(s) -
Rondini Elizabeth A,
Gardner Elizabeth M,
Fenton Jenifer I
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.977.5
Subject(s) - granzyme , granzyme b , cytolysis , colitis , biology , immunology , lymphocyte , granzyme a , immune system , cd3 , cd8 , cytotoxic t cell , perforin , biochemistry , in vitro
Increasing evidence suggests an important role for natural killer (NK) cells in providing innate defense against pathogenic bacteria. We reported that feeding fish oil enriched with decosahexaenoic acid (DFO) exacerbated colitis and promoted colonic dysplasia in SMAD3−/− mice following infection with H. hepaticus . Flow cytometric analysis of secondary lymphoid tissues suggested alterations in NK cell populations. The objective of this study was to determine the effect of DFO on cytolytic lymphocyte infiltration at the local site of inflammation/dysplasia by immunohistochemistry. Mice were fed AIN‐93G diets modified to include corn oil (CO) or DFO (0.75–6.0 %) as the fatty acid source for 8 wks, gavaged with H. hepaticus to induce colitis, then euthanized 4 wks post‐infection. Colons were removed and processed for analysis of cytolytic cells (DX5 and CD3) as well as the granzyme B by immunohistochemistry. We found a progressive increase in tumor associated CD3+ and DX5+ lymphocytes as well as granzyme B staining as dietary DFO increased, consistent with increased colitis scores. However, despite significant infiltration of cytolytic cells in the 6% DFO group, the intensity of granzyme B staining was reduced. These data imply that high levels of DFO may impair activation of cytolytic lymphocytes potentially contributing to accelerated tumor formation previously reported. Grant Funding Source : n/a