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Zinc deficiency increases miR‐34a expression in mice
Author(s) -
Liuzzi Juan,
Valencia Katherine,
Cao Jay,
Gonzalez Armando
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.977.1
Subject(s) - zinc deficiency (plant disorder) , microrna , zinc , biology , gene expression , suppressor , regulation of gene expression , small intestine , microarray , gene , microbiology and biotechnology , endocrinology , genetics , chemistry , organic chemistry
MicroRNAs play a significant role in the regulation of gene expression in a plethora of eukaryotic systems by binding to complementary regions of specific target mRNAs. To explore the possible changes of microRNA expression induced by zinc deficiency, we compared the global microRNA expression in small intestine of young CD‐1 mice fed either a zinc‐sufficient or zinc‐deficient diet. Microarray analysis revealed up‐regulation of miR‐34a, miR‐1949, miR‐1274a, and miR‐140 in small intestine of zinc deficient mice. Validation study using RT‐PCR showed that zinc deficiency increased miR‐34a expression in small intestine and thymus but did not alter the expression of this microRNA in liver. Moreover, zinc deficiency did not affect the expression of the miR‐34a target SIRT1. The miR‐34 family has been implicated in the p53 tumor suppressor network. Therefore, the up‐regulation of miR‐34a associated with zinc deficiency could be part of an adaptive response to oxidative DNA damage caused by zinc depletion. This project was supported by Florida International University.