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CMO‐II KO mice display altered lipid metabolism compared to CMO‐I KO and wild‐type mice
Author(s) -
Elsen Amy Christine,
Ford Nikki Ann,
Erdman John W
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.975.9
Subject(s) - medicine , endocrinology , lipid metabolism , metabolism , adipose tissue , chemistry , adipocyte , wild type , biology , biochemistry , gene , mutant
Adult mice lacking the β‐carotene central cleavage enzyme, carotene‐15,15'‐monoxygenase (CMO‐I), have altered lipid metabolism, but little is known about the lipid metabolism in carotene‐9′10′‐monooxygenase II knock‐out (CMO‐II KO) mice. The objective of this study was to examine lipid metabolism in female CMO‐I KO, CMO‐II KO and wild type (C57BL/6Jx129x1/SvJ) mice fed AIN‐93G based diets for 30 days: 20mg lycopene/kg diet from lycopene beadlets, 10% tomato powder, or placebo beadlets. CMO‐II KO mice were significantly smaller (p <.0001) and gained less weight (p <.0001) compared to CMO‐I KO and wild‐type mice. Hepatic lipid content of CMO‐II KO mice was significantly greater compared to CMO‐I KO and wild‐type mice (p <0.05), but serum cholesterol levels were lower in both CMO‐II KO and CMO‐I KO mice compared to wild‐type mice (p <.0001). Expression of adipogenic genes, PPAR‐γ and Acox‐1 was measured in liver and adipose tissue. Hepatic PPAR‐α and adipocyte protein 2 were also measured. Differences were primarily seen between genotypes suggesting that diet did not affect gene expression. Adipose PPAR‐γ expression was greater in CMO‐II KO mice (p <.0001), whereas ap2 gene expression was lower (p <0.05). These results suggest that CMO‐II KO mice have altered lipid metabolism compared to CMO‐I KO mice and wild‐type mice. (Funded in part by NIH grant PHS‐1‐RO1 CA125384.) Grant Funding Source : NIH