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Rapid method for measuring carotenoid and retinoid concentration in blood and breast milk for dietary intervention assessments
Author(s) -
Burri Betty J,
Turner Tami,
Haskell Marjorie,
Jamil Kazi,
Jamil Maleka
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.975.18
Subject(s) - breast milk , bioavailability , carotenoid , breastfeeding , lutein , medicine , repeatability , vitamin , vitamin a deficiency , breast feeding , food science , zoology , physiology , chemistry , retinol , biology , chromatography , pediatrics , pharmacology , biochemistry
Vitamin A deficiency (VAD) is a major public health problem in many developing countries. β‐carotene (BC) and β‐cryptoxanthin (CX) can be converted to VA and are important sources of this nutrient. CX appears to have higher bioavailability than current estimates and may be an unappreciated source of VA. We are assessing the impact of dietary interventions with foods rich in CX and BC on plasma and breast milk concentrations of VA, BC, and CX. We randomly assigned exclusively breastfeeding women to receive a VA supplement, tangerines, orange sweet potatoes, or white potatoes 6 days a week for 3 weeks. Concentrations of CX, BC, and VA are measured in plasma and breast milk at baseline and after 3 weeks. Due to the large number of samples in this study, we compared extraction methods, and developed a fast isocratic reverse‐phase HPLC method to analyze VA, BC, CX, and other carotenoids in biological samples. Run times were complete in less than nine minutes. Repeatability was between 1.0 and 5.1 percent (RSD) within and between day trials except for lutein and zeazanthin, that co‐elute. Limits of detection were between 1.3 and 15 ηg/ml. These methods provide efficient processing of large quantities of blood and breast milk for the analysis of carotenoids and retinoids. Supported by AFRI/NIFA Grant 2008‐01889.