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Effect of age and body weight on expression and serum concentration of GH, IGF‐1, and IGFBP in pigs
Author(s) -
Trejo Adriana Morales,
Avelar Ernesto,
Cervantes Miguel,
Araiza Alfonso B.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.972.5
Subject(s) - medicine , endocrinology , body weight , blood sampling , biology
There are no available reports regarding the effect of age and body weight (BW) of pigs on the expression of GH, IGF‐1, and IGFBP. A study was conducted to analyze the relationship between age and BW with serum concentration (SC) of GH and IGF‐1, and relative expression (RE) of GH and IGFBP in pigs. Twenty pigs were sacrificed; 4 at each of the following ages: 1, 30, 60, 120, and 180 d. Samples from hypophysis and liver were collected after pigs were sacrificed, total RNA was purified, and cDNA was synthesized to carry out real time qPCR for GH and IGFBP; 18S rRNA was used for normalization. Twenty more pigs were used to collect blood samples at the ages indicated above; serum was collected to analyze GH and IGF‐1 using ELISA. SC of GH and IGF‐1 at each sampling age were: 16.2, 6.9; 23.0, 12.2; 21.0, 12.8; 19.7, 17.6; and 20.4, 14.7 ng/ml, respectively. RE of GH in hypophysis at each age was: 833, 309, 629, 168, and 76, arbitrary units, respectively; in liver, RE of IGFBP was: 21.3, 9.0, 8.5, 3.8, and 6.7, respectively. SC of GH quadratically (P<0.05) and IGF linearly (P<0.01) increased with age and BW. RE of GH (P<0.01) and IGFBP (P<0.01) linearly reduced with age and BW. There were negative correlations between RE and SC of GH (P<0.05), RE of GH and SC of IGF‐1 (P<0.01), and RE of IGFBP and SC of IGF‐1 (P<0.01). These data show a significant and differentiated effect of age and BW on GE and SC of GH, IGF‐1, and IGFBP. (Financial support by CONACyT, México).