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Lutein and Zeaxanthin Supplementation Suppresses Ocular and Systemic Inflammatory Response
Author(s) -
Shang Fu,
Bian Qinging,
Gao Shasha,
Qin Tingyu,
Ren Zhihong,
Wu Dayong,
Taylor Allen
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.95.6
Subject(s) - lutein , zeaxanthin , lipopolysaccharide , inflammation , tumor necrosis factor alpha , xanthophyll , interleukin 6 , pharmacology , chemistry , immunology , endocrinology , medicine , biochemistry , carotenoid
The objective of this work is to determine the effects of lutein and zeaxanthin supplementation on ocular and systemic inflammatory response. ARPE‐19 cells and primary marine macrophages were cultured in the presence of 0, 1 and 10 μM lutein or zeaxanthin for 1–3 days and then stimulated with lipopolysaccharide (LPS) for 8 h. Levels of IL‐6, IL‐8 and TNFα in the medium were determined by ELISA and used as indicators of inflammatory response. To test the anti‐inflammatory effects in vivo, C57/BL mice were fed diets containing 0, 0.02% and 0.1% lutein or zeaxanthin for 3 months. Levels of IL‐6 and TNFα secreted by macrophages from these mice were determined. Treatment of RPE and macrophages with LPS resulted in a dramatic increase in production of IL‐6, IL‐8 and TNFα. Supplementation of lutein suppressed LPS‐stimulated production of these inflammatory mediators in both RPE and macrophages in a dose‐dependent manner. Furthermore, macrophages isolated from lutein supplemented mice produced less IL‐6 and TNFα upon LPS stimulation and the suppressive effects were also dose‐dependent. Supplementation of zeaxanthin suppressed the production of IL‐8, but not IL‐6. These data indicate that lutein and zeaxanthin have anti‐inflammatory effects and that increased dietary lutein or zeaxanthin intake may reduce the risk for AMD via modulating ocular and systemic inflammation. Supported by USDA AFRI Award 2009‐35200‐05014, NIH grant EY 011717, USDA contract 1950‐510000‐060‐01A, and Dennis L. Gierhart Charitable Gift.