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Aberrant expression of Glia Maturation Factor in Human PD brain
Author(s) -
Zaheer Asgar,
Thangavel Ramasamy,
Anantharam Poojya,
Wynn DonRaphael,
Sahu Shailendra K,
Zaheer Smita
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.945.13
Subject(s) - microglia , neurodegeneration , substantia nigra , parkinson's disease , pathogenesis , human brain , autopsy , pathology , dopaminergic , downregulation and upregulation , neuroscience , neuroinflammation , immunohistochemistry , midbrain , medicine , biology , disease , central nervous system , immunology , inflammation , gene , dopamine , genetics
Glia maturation factor (GMF) is an inflammatory protein, discovered in our laboratory, plays a major role in the pathogenesis of CNS inflammatory diseases. In the present study we demonstrate a significant up regulation of GMF in human PD brain. Human brain tissues were obtained from autopsy at University of Iowa Deeded Body Program. For the comparison, we examined brain tissue from five cases with clinically diagnosed and neuropathologically confirmed Parkinson's disease and six age‐matched non‐PD controls. Representative images showing degenerating TH+ neurons/fibers associated with activated microglia and astrocytes in the autopsied brain from PD case compared with age‐matched non‐PD controls. In the substantia nigra of patients with PD, GMF appears to be localized to astrocytes and dopaminergic neurons. We also estimated GMF protein levels in PD brains. We demonstrated a significant increase (over 10 fold) in GMF concentration in the SN and STR of PD patients compare to non‐PD controls. Thus, our results provide strong evidence that under conditions of neurodegeneration in PD the expression of GMF is significantly upregulated. (Supported by VA Merit Review award and NIH/NINSD grant NS‐47145)