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Analysis of a simulated in vivo environment for selective induction of apoptosis
Author(s) -
Dover Cheri Denise
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.943.9
Subject(s) - apoptosis , propidium iodide , in vivo , population , subculture (biology) , camptothecin , microbiology and biotechnology , programmed cell death , cancer cell , cell , chemistry , cell culture , viability assay , biology , biochemistry , genetics , cancer , medicine , environmental health
Apoptosis is thought to play a major role in biological processes and in establishing effective treatments for cancer. The objective of this study was to examine the response of human erythroleukemia (HEL) cells when exposed to known apoptosis inducers and to analyze a simulated in vivo environment to determine whether normal cells (WI‐38 cells) and HEL cells were affected by the chemicals. The HEL cells were treated with camptothecin (CP), vehicle alone, and various heteroarotinoids (HETS) twenty‐four hr after subculture and incubated for an additional 48–72 hr. The cells were harvested and monitored for apoptosis, growth, and viability. Apoptosis was quantitated using the Hoechst/propidium iodide assay. Preliminary results proved one of the five HETS tested had significant elevated levels of apoptosis when compared to the vehicle alone. In an attempt to mimic an in vivo environment, HEL cells, grown in suspension, were introduced to a normal human adherent cell population at various cell densities and allowed to incubate for 72 hr. The majority of the HEL cells adhered to the WI‐38 cells. A large population of HEL cells was resistant to removal from the WI‐38 cells following a vigorous shake, suggesting a strong bond between the cells. In order to determine the selectivity of apoptosis, CP was added to the system. The initial results suggest that both cell types were negatively affected by CP. Support was provided by SRU.