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Adipose tissue triglyceride lipase mRNA is present in the small intestine and increased in response to acute and chronic high fat feeding in mice
Author(s) -
Uchida Aki,
Whitsitt Mary C.,
Lee Bonggi,
Slipchenko Mikhail N.,
Cheng JiXin,
Buhman Kimberly K.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.936.4
Subject(s) - adipose triglyceride lipase , medicine , endocrinology , triglyceride , adipose tissue , monoacylglycerol lipase , lipase , messenger rna , fatty acid , small intestine , chemistry , lipid droplet , biology , lipolysis , biochemistry , enzyme , cholesterol , gene , receptor , endocannabinoid system
Adipose triglyceride lipase (ATGL or PNPLA2) specifically catalyzes the initial removal of a fatty acid from triacylglycerol (TG) to generate diacylglycerol and fatty acid. Recent studies show that ATGL is highly abundant in adipose and liver tissue and critical for TG turnover. However, expression and regulation of ATGL in the small intestine is unknown. We determined that ATGL mRNA is expressed along the length of the small intestine at similar levels. To determine whether ATGL mRNA levels are regulated by acute high fat feeding, we measured mRNA levels in the jejunum of mice fed 200 μl olive oil via oral gavage. We found that ATGL mRNA levels were higher two hours post gavage and returned to control levels by four hours post gavage. Similarly, enterocyte TG storage observed by coherent anti‐Stokes Raman scattering microscopy found higher TG storage at 2 hours post gavage and decreased TG storage at 4 hours post gavage. We also observed that mice fed a high fat diet ad libitum for 3‐weeks had a 4.3 fold higher level of ATGL mRNA in the jejunum. Our results indicate that ATGL mRNA levels are regulated acutely and chronically by dietary fat and correlates with TG storage in enterocytes.