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Denervation increase Akt phosphorylation and reduce glyceroneogenesis in white adipose tissue from diabetic rats
Author(s) -
Frasson Danubia,
Chaves Valéria Ernestânia,
Garófalo Maria Antonieta Rissato,
Navegantes Luiz Carlos Carvalho,
Migliorini Renato Hélios,
Kettelhut Isis do Carmo
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.936.1
Subject(s) - white adipose tissue , medicine , endocrinology , denervation , phosphoenolpyruvate carboxykinase , protein kinase b , adipose tissue , insulin , chemistry , diabetes mellitus , lipolysis , phosphorylation , biochemistry , enzyme
It has been known that adipose tissue (WAT) has three sources of glycerol‐3‐phosphate (G3P) generation and these pathways seem to be reciprocally controlled to maintain the stores of TAG. The aim of this work was to understand the role of SNS and insulin in these processes. WAT from diabetic and controls rats was denervated and in vitro glucose uptake, P‐ enol ‐pyruvate carboxykinase (PEPCK) activity, a key enzyme of glyceroneogenesis and Akt phosphorylation were evaluated. Chemical denervation (D) of epididymal WAT was performed 7 days before the experiments, using contralateral WAT as control. Diabetes was induced by i.v. STZ injection (40mg/Kg) 72h before the experiments. D caused 88% reduction in WAT NOR content. Diabetes reduced the in vitro glucose uptake (nmol. 10 6 cells −1 .min −1 ) (1.1±0.1 vs control 1.5±0.03) and D caused a further reduction in adipocytes of diabetic rats (0.5±0.1 vs innervated(IN) 1.1±0.1). Diabetes increased PEPCK activity (nmol.mg protein −1 .min −1 ) (12.9±1.1 vs control 2.6±0.1) and PEPCK content (70%), D reduced PEPCK activity (4.0±0.4 vs IN 12.9±1.1) and also PEPCK content (30%). Diabetes reduced pSer 473 ‐Akt (38%) and D increased Akt phosphorylation by 69%. The data suggest that SNS can regulate glyceroneogenesis and denervation seems to increase the insulin response in diabetic animals. Financial support: CNPq and FAPESP.