Premium
PLD has a role in the formation of clathrin‐coated vesicles and in the structure maintenance of Golgi apparatus in HSY cells
Author(s) -
Souza Lorena Brito,
Jamur Maria Célia,
Oliver Constance
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.934.1
Subject(s) - golgi apparatus , phosphatidic acid , phospholipase d , pld2 , endocytosis , microbiology and biotechnology , vesicle , clathrin , intracellular , chemistry , biology , biochemistry , cell , endoplasmic reticulum , phospholipid , signal transduction , membrane
Phospholipase D (PLD) hydrolyses phosphatidylcholine to produce phosphatidic acid (PA) and choline. PLD has been implicated in many cellular functions, however, the function and intracellular localization of PLD varies according to cell type. The aim of this study was identify the intracellular distribution of the isoforms PLD1 and PLD2 and determine the role of PLD in the formation of clathrin‐coated vesicles (CCVs) from Golgi apparatus in duct cells of parotid gland. Treatment of cells with the primary alcohol 1‐butanol (1‐ButOH) inhibits the hydrolysis of phosphatidylcoline by PLD thereby suppressing phosphatidic acid (PA) production. Instead, phosphatidyl‐1‐butanol is formed by a transphosphatidylation reaction. In untreated HSY cells, there is a co‐localization of PLD with CCVs. When the cells were incubated with 1‐ButOH, clathrin‐coated vesicles, PLD1 and PLD2 are recruited to the perinuclear region. The decrease in PA levels after treatment with 1‐ButOH also results in an accumulation of lysosomes in the perinuclear region and affects the Golgi apparatus, increasing the volume of the Golgi saccules. Therefore, PLD is involved in the formation of Golgi associated CCVs as well as in the structural maintenance of the Golgi apparatus in HSY cells. FAPESP, CNPq, CAPES