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PDGF‐BB induces the cell surface co‐localization of laminin and dystroglycan and fibronectin and α5‐integrin
Author(s) -
Kastanis George John,
HernandezNazara Zamira,
Nieto Natalia,
RincónGallardo Ana Rosa,
Popratiloff Anastas,
DominguezRosales Jose Alfredo,
Lechuga Carmen,
Rojkind Marcos
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.930.8
Subject(s) - laminin , fibronectin , integrin , microbiology and biotechnology , platelet derived growth factor receptor , cell migration , chemistry , receptor , extracellular matrix , messenger rna , biology , cell , growth factor , gene , biochemistry
Hepatic stellate cells (HSC) are embedded in a laminin‐rich matrix in the Space of Disse of the normal liver. During injury HSC migrate to the injured sites and produce scar collagen. One of the first proteins produced during liver injury is fibronectin. Therefore we investigated the role of laminin, fibronectin and its receptors in HSC migration. METHODS We used mouse HSC treated with PDGF‐BB and used Northern and Western analysis to study the expression of dystroglycan (DG) and α5‐integrin (α5‐Int), the respective receptors for laminin (LN) and fibronectin (FN). We also used confocal microscopy to investigate the cellular localization of DG and α5‐Int. RESULTS PDGF‐BB induced the cell surface colocalization of α5‐Int and FN as well as DG and LN. While DG and LN were localized to the projections of the HSC, FN and α5‐Int were present along the body of the cells. PDGF‐BB also induced significant changes in the expression and regulation of DG and α5‐Int. While the expression of α5‐Int was up‐regulated that of DG and LN was down‐regulated. However, DG RNA levels were sustained due to the increased transcription of the gene with a 50% decrease in its mRNA t 1/2 . Antibodies to DG or α5‐Int prevented PDGF‐BB–induced HSC migration. CONCLUSION Overall, our findings suggest that both LN and FN are required for HSC migration. However, while the cells migrate on a FN carpet, they appear to be anchoring on LN.