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Identification of novel long non‐coding RNA candidates in male germ cell development
Author(s) -
LEE TINLAP,
Xiao Amy,
Chan WaiYee,
Rennert Owen M
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.924.3
Subject(s) - biology , germ cell , gene , spermatogenesis , non coding rna , developmental biology , rna , genetics , computational biology , long non coding rna , germline , male infertility , genome , infertility , pregnancy , endocrinology
Male factor accounts for at least 40% of infertility cases. A large number of cases are attributed to a failure of spermatogenesis, a complex developmental process that gives rise to mature spermatozoa. Despite advances in genomics and molecular biology, previous attempts to delineate the developmental programs in spermatogenesis have had limited successes. This is largely because the focus is limited to protein‐coding genes. A significant portion of genome actually encodes many transcripts known as non‐coding RNAs (ncRNAs) of unknown functions. Long ncRNAs (lncRNAs) are generally classified as ncRNAs with more than 200 base pairs. They have been recently demonstrated to involve in developmental regulations in stem cell, brain and cancer. The lncRNA candidates and its functional importance in male germ cell development are not known. Based on our previous established Serial Analysis of Gene Expression (SAGE) libraries for male germ cell development, we have identified a list of lncRNA candidates that demonstrate specific expression in the major developmental stages, including type A spermatogonia, pachytene spermatocytes and round spermatids. The data provides a new and substantially different approach in addressing novel developmental mechanisms in spermatogenesis.