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Folic acid supplementation inhibits NADPH oxidase‐mediated superoxide anion production in the kidney
Author(s) -
Hwang SunYoung,
Siow Yaw L,
AuYeung Kathy K.W.,
House James,
Karmin O
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.917.2
Subject(s) - superoxide , nadph oxidase , oxidative stress , xanthine oxidase , homocysteine , hyperhomocysteinemia , nox4 , methionine , medicine , endocrinology , chemistry , kidney , superoxide dismutase , lipid peroxidation , oxidase test , biochemistry , enzyme , amino acid
Hyperhomocysteinemia (HHcy), a condition of elevated blood homocysteine (Hcy) levels, is a metabolic disease. Our recent studies indicate that HHcy can lead to renal injury by inducing oxidative stress. Oxidative stress is one of the important mechanisms contributing to Hcy‐induced tissue injury. Folic acid supplementation is regarded as a promising approach for prevention and treatment of cardiovascular disease associated with HHcy due to its Hcy lowering effect. However, its effect on the kidney is not clear. The aim of this study was to examine the effect of folic acid supplementation on Hcy‐induced superoxide anion production via NADPH oxidase in the kidney. HHcy was induced in male SD rats fed a high‐methionine diet for 12 wk with or without folic acid supplementation. There was a significant increase in NADPH oxidase‐mediated superoxide production and lipid peroxidation in HHcy rats. Folic acid supplementation effectively antagonized HHcy‐induced oxidative stress. Inhibitory effect of folic acid on NADPH oxidase activation was mediated through inhibition of NOX4 and p22phox expression. In addition, folic acid supplementation attenuated xanthine oxidase activity and restored SOD activity in the kidney. These results suggest that folic acid supplementation may offer renal protective effect against oxidative stress. (This study was supported by NSERC)