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ANTIOXIDANTS DECREASE LIPOLYSIS AND LIPID SYNTHESIS IN HUMAN DIFFERENTIATED ADIPOCYTES
Author(s) -
Krawczyk Sarah Anne,
Deeney Jude T.,
Corkey Barbara E.
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.914.5
Subject(s) - lipolysis , chemistry , triglyceride , basal (medicine) , medicine , antioxidant , glycerol , endocrinology , lipid droplet , reactive oxygen species , forskolin , adipose tissue , lipid metabolism , biochemistry , in vitro , biology , insulin , cholesterol
OBJECTIVE Reactive oxygen species (ROS) partially mediate the aberrant effects of chronic inflammatory states such as obesity. The goal of this research was to examine the effect of ROS on triglyceride synthesis and lipolysis by scavenging ROS with the antioxidant, diphenyliodonium (DPI). METHODS Human subcutaneous preadipocytes were differentiated for 12 days into adipocytes. Cells were then treated with DPI and labeled with tritiated oleate (3H‐FA) or 32P as a tracer to assess lipid synthesis, which was detected by thin layer chromatography (TLC). Glycerol release was measured using an enzyme‐linked assay. Protein was assessed using western blot. RESULTS There was a decrease in glycerol released into the media of cells treated with 10 μM DPI for either 1 or 4 hours, as compared to control cells under basal and forskolin‐stimulated conditions. In parallel, triglyceride (TG) synthesis was decreased with DPI treatment compared to control (for basal and stimulated conditions). A preliminary look at the PKA pathway by western blot showed no change in activation compared to control by DPI treatment. CONCLUSIONS Data indicate that both 1 and 4 hour treatment with DPI decreased basal lipolysis and concomitantly decreased neutral lipid synthesis. This may be due to decreased TG synthesis, decreased lipolysis, or a decrease in turnover.