Premium
Novel transcript variants arise from alternate promoter usage and alternative splicing in 5′ regions of cAMP‐dependent Protein kinase A subunit genes in mouse
Author(s) -
Banday Abdul Rouf,
Azim Shafquat,
Tabish Mohammad
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.900.3
Subject(s) - exon , gene , alternative splicing , biology , untranslated region , coding region , genetics , rna splicing , protein subunit , cloning (programming) , microbiology and biotechnology , messenger rna , rna , computer science , programming language
Protein kinase A (PKA) is composed of four membered structure with two regulatory (R) and two catalytic (C) subunits. There are four different R subunits (Iα and Iβ, IIα and IIβ) and two different C subunits (Cα and Cβ) in mouse, each encoded by separate gene. Earlier, few alternative spliced variants have been identified with tissue specific function. The objective of this study was to analyze splicing events in 5′ region of R and C subunit genes of PKA in mouse. Analysis was done with the help of combinatorial approach of bioinformatics tools and molecular biology techniques like gene and exon prediction programmes, ORF finders, EST searches, alignment tools, 5′ RACE, RT‐PCR, sub‐cloning, sequencing etc. We were able to sequence two types of cDNA clones from mouse tissues, one type having alternative 5′ untranslated exon (s) and other type having alternative 5′ first coding exon. In former the variants arise either from alternate promoter usage or splicing of 5′ untranslated exons and in later new exon replaces the canonical first coding exon to exhibit different N‐terminal to translated protein. However, alternate promoter usage is mostly linked to regulatory subunit genes. The numbers of new transcript variants sequenced were four in Cα, two in Cβ, three in R Iα and four in R Iβ. Their expression profiles showed variation across the tissues together at different development stages. The research was funded by DBT, India.