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miR‐709 regulates miR‐15a/16 biogenesis at post‐transcriptional level in nucleus: an implication of a microRNA hierarchy system
Author(s) -
Tang Rui,
Li Limin,
Cao Tin,
Hou Dongxia,
Chen Junyuan,
Zhang Chenyu,
Zen Ke
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.899.4
Subject(s) - microrna , nucleus , biogenesis , microbiology and biotechnology , biology , cell nucleus , messenger rna , cell , gene , genetics
MicroRNAs are endogenous non‐coding RNAs of 21–23 nt which regulate target gene expression at the posttranscriptional level typically through either translational repression or mRNA cleavage. Recent developments have revealed that there is extensive of miRNAs as well as miRNA pathways related proteins in cell nucleus, leading us to hypothesize that nucleus microRNAs might have their own functions. We observed that a specific microRNA, miR‐709, is detected in nucleus fraction preparing from various mouse cell lines as well as tissues samples. In cell nucleus miR‐709 will meet and bind its target, pri‐miR‐15a/16‐1, which is a primary transcript for another microRNA cluster: miR‐15a and miR‐16. Such binding will prevent pri‐miR‐15a/16‐1 processing in nucleus and thus regulate miR‐15a/16 maturation at the pri‐miRNA to pre‐miRNA stage. Furthermore, nucleus miR‐709 participates cell apoptosis through BCL2‐miR‐15a/16 pathway. Our data indicates that one microRNA can directly target another microRNA's primary transcript in nucleus and regulate its biogenesis via this pathway. In conclusion this association may further suggest a “microRNA hierarchy” system in biological world.