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MicroRNAs during Murine Molar Tooth Development
Author(s) -
Osmundsen Harald,
Sehic Amer,
Jevnaker AnneMarthe
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.899.3
Subject(s) - antagomir , microrna , biology , microarray , molar , gene , microarray analysis techniques , gene expression , blot , messenger rna , microbiology and biotechnology , fold change , genetics , paleontology
MicroRNAs (miRNAs) may have essential functions as regards regulation of gene expression, and are likely involved also during development. We have mapped miRNAs during development of the murine first molar tooth germ. The miRNA expression profile was found highly dynamic. During late odontogenesis miR‐214 is highly expressed, and was selected for further studies using antagmomir‐214. Antagomir‐214 was given as mandibular injections (0–100 pmol) at postnatal day 1 (P0). First molar tooth germs were removed at various times. Levels of miRNAs and mRNAs were measured using microarrays. A dosage of 50 pg was found to be optimal. At 24 h after injection the level of miR‐214 was about 0.02‐fold of control level, the level of expression returning close to the control at 72 h. Using a dosage of antagomir‐214 of 50 pmol about 1200 mRNAs and 8 miRNAs were differentially expressed in the tooth germ 24 hrs after injection of antagomir‐214. The microarray results were validated using RT‐PCR. Western‐blotting data was in agreement with changes mRNA levels. Differentially expressed genes were subjected to bioinformatic analysis, suggesting that cellular functions and signaling pathways were significantly altered. We conclude that miR‐214 may have significant regulatory functions during late odontogenesis. Project funding from Dental Faculty, University of Oslo.