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Transcriptional Regulation by the Ccr4/Not4 Complex
Author(s) -
Mehrotra Swati,
Moon John,
Vancura Ales
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.893.4
Subject(s) - transcriptional regulation , chromatin immunoprecipitation , histone , biology , acetylation , psychological repression , regulation of gene expression , gene , histone h3 , histone methyltransferase , promoter , hdac4 , chromatin , gene expression , microbiology and biotechnology , genetics
The CCR4/NOT complex is a global regulator of gene expression that affects expression both positively and negatively. The complex, conserved from yeast to human, is composed of at least nine subunits. The Ccr4 subunit functions in the control of mRNA deadenylation and transcriptional initiation/elongation. The NOT proteins are involved in transcriptional repression by restricting access of TFIID to promoters. The CCR4‐NOT complex also plays a key role in sensing nutrients and stress. We observed increased expression of stress responsive genes HSP12 , HSP26 and CTT1 in ccr4 Δ and not4 Δ strains. The aim of this study was to elucidate the mechanism of transcriptional regulation of the stress responsive genes in ccr4 Δ and not4 Δ strains. Western blot and chromatin immunoprecipitation revealed that ccr4 Δ and not4 Δ cells display a decreased level of histone acetylation that is not confined to specific loci but is global in distribution. The increased expression of stress responsive genes HSP12 , HSP26 and CTT1 in ccr4 Δ and not4 Δ strains can be partially suppressed by mutations in histone deacetylases HDA1 and RPD3 . The results implicate Ccr4/Not complex in regulation of histone acetylation and are consistent with a model in which Ccr4/Not complex‐supported histone acetylation is required for repression of stress‐inducible genes.