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Novel Alternative and Multistranded DNA Microarrays: Z‐DNA, Triplex DNA and Quadruplex DNA
Author(s) -
Gagna Claude Eugene,
Dannibale Robert,
Beheshti Kayvan,
Demorcy Ashley,
Lui Pearl,
Yadiapalli Divya,
Pasquarella Anthony V,
Rehmat Shabia,
John Akaz,
Okereke Gloria,
Chiang Goretti,
Marler Shanacy,
Nanda Priya,
Syed Sarah,
Kohli Sahil,
Malik Shehryar,
Rahman Sabreen,
Patel Aney,
Joy Job,
Lambert W Clark
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.887.1
Subject(s) - dna microarray , dna , biology , nucleic acid , gene , microbiology and biotechnology , computational biology , genetics , gene expression
At the ends of chromosomes are segments of DNA called telomeres, which permit cells to replicate, protect DNA, and repair DNA. Multistranded and alternative DNA structures play important roles in gene expression. Novel alternative, multistranded, and helical transitional nucleic acid microarrays have been constructed, viz., Z‐DNA, triplex DNA and quadruplex DNA. These microarrays allow for characterization of the structure and function of Z‐DNA, triplex DNA and quadruplex DNA under different conditions. They allow for identification of drugs that bind to multistranded nucleic acids and for characterization of genomic elements that contain triplex DNA and telomeres. The novel microarrays will allow for a new approach towards studying gene expression and drug discovery. The new microarrays go beyond the limitations of conventional DNA microarrays, which focus on the primary structure of nucleic acids (i.e., base pairs) and ignore DNA secondary structure. These microarrays can be used for aging, cancer and infectious disease (e.g., tuberculosis, malaria and AIDS) research. The microarrays can also be employed for enhancing or inhibiting of gene expression. With these next generation DNA microarrays, scientists will have access to all of the uncharted structures that control gene expression, and aid in developing new classes of drugs for disease. Supported by a 2010 NYIT ISRC grant.