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The nuclear matrix protein, Matrin 3 is required export of HIV‐1 unspliced/partially spliced RNAs
Author(s) -
Yedavalli Venkat,
Jeang KuanTeh
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.886.3
Subject(s) - rna splicing , nuclear export signal , rna , microbiology and biotechnology , biology , rna binding protein , translation (biology) , cytoplasm , messenger rna , gene , cell nucleus , chemistry , genetics
One central aspect of gene regulation and cell functions is post‐transcriptional regulation of transcripts. Post‐transcriptional regulation involves splicing, nucleo‐cytoplasmic export of spliced RNA, stabilization‐degradation, and the translation of transcribed mRNAs. For HIV‐1, complexities exist because a single unspliced RNA must direct the synthesis of viral proteins and also serves as the genomic RNA for progeny virions. Thus unlike cellular RNAs, viral RNAs that are unspliced or partially spliced must be able to exit the nucleus and progress into the cytoplasm. To accomplish this task, HIV‐1 encodes a viral protein Rev which binds to a cis‐RNA element, RRE, which is contained on unspliced and incompletely spliced viral RNA. Rev acts to direct the export of RRE‐containing viral RNAs, and Rev has been shown to interact with cellular proteins CRM1, DDX3, PIMT and others to mediate their export. Here, we report the identification of a nuclear matrix protein, Matrin 3, as a key co‐factor which mediates Rev‐dependent expression. We discuss proposed mechanisms for how Matrin 3 acts to assist Rev function. Research supported by Intramural funds from NIAID, and by the Intramural AIDS Targeted Antiviral Program (IATAP) from the office of the Director, NIH