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Identification of genes regulating recombination of repetitive DNA
Author(s) -
Chadha Akash,
Lee Thomas,
Scheifele Lisa
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.879.1
Subject(s) - flp frt recombination , biology , transposable element , retrotransposon , genetics , homologous recombination , recombination , ectopic recombination , gene , mitotic crossover , non allelic homologous recombination , genetic recombination , dna , recombinase , cre lox recombination , site specific recombination , genome , transgene , genetically modified mouse
Transposons mold the genome by transposition and through subsequent recombination between the dispersed repeats. Recombination between Ty1 elements can produce chromosome aberrations in yeast. We previously developed a unique set of Retrotransposon Overdose (RO) strains which contain 3 to 10‐fold more Ty1 elements (Scheifele, PNAS 2009). We used RO strains to screen for genes controlling Ty1 recombination and identified DNA replication, repair, and cohesion genes that, when deleted, produce growth defects specific to RO strains. To confirm that these growth defects are due to increased recombination, we used a strain of yeast with a marked Ty1 element, individually deleted the genes identified in our screen, and measured the rate of marker loss; deletion of genes that control Ty1 recombination should increase loss of the genetic marker. Further, by comparing the frequency of marker loss, we can determine which genes have the greatest role in preventing recombination between Ty1 elements. While most of the gene deletions increased Ty1 recombination, the magnitude of their effects varied. In addition, we observed variation in the type(s) of recombination affected, with differential effects on recombination between transposons, between direct repeats, and between lys2 heteroalleles. This specificity suggests that there may be distinct mechanisms to regulate recombination between repetitive DNA sequences.