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Elastase – proteolytic stimulus leads to redox imbalance in the early stage of pulmonary response
Author(s) -
Lanzetti Manuella,
Pires Karla Maria P,
Barroso Marina V,
Nesi Renata T,
Lopes Alan A,
Alves Jackson N,
Victoni Tatiana,
Benjamim Claudia,
Lagente Vincent,
Valenca Samuel S,
Porto Luis Cristovao
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.877.4
Pulmonary emphysema has become epidemic worldwide. Both inflammatory and oxidative processes are crucial to parenchyma destruction. We investigate the participation of oxidative stress in elastase‐induced emphysema in mice. The trachea of C57BL/6 mice were instilled with pancreatic porcine elastase (PPE 0.5U or PPE 3U). Lung tissue was collected after 1, 7, 14 and 21days for morphometry and homogenates were analized for catalase (CAT), superoxide dismutase (SOD) and myeloperoxidase (MPO) activities. TNF‐α, IL‐10, nitrite and TBARS levels were measured in BAL. Emphysema typical morphological alterations were seen at 21 days after PPE 0.5U (Table 1). TNF‐α was elevated at 7 and 14 days after PPE 0.5U with a reduction in IL‐10 levels. MPO was elevated with PPE 0.5U. The levels of Nitrite, TBARS and the SOD activity were increased at 7 days after PPE 0.5U, while CAT activity was decreased. PPE‐induced emphysema also results in oxidative stress. This redox imbalance generated by may be crucial in the early stage of the injury and has no effect in later stages. Grant Funding Source : CAPES/COFECUB