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The spectrin cytoskeleton is an integral target and crucial component of adherent, invasive triggering and invasive zippering bacterial diseases
Author(s) -
Ruetz Tyson Joel,
Cornick Steve,
Guttman Julian Andrew
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.876.3
Subject(s) - spectrin , cytoskeleton , listeria monocytogenes , microbiology and biotechnology , biology , actin , actin cytoskeleton , cytoplasm , cell , genetics , bacteria
Entropathogenic Escherichia coli (EPEC), Salmonella Typhimurium ( S. Typhimurium), and Listeria monocytogenes ( L. monocytogenes ) all manipulate the host cell cytoskeleton at crucial stages of their disease processes. Although the actin cytoskeleton has been previously identified as a ubiquitous target of these pathogens, the examination of the spectrin cytoskeleton has been largely overlooked. Here we show that spectrin and the spectrin‐associated proteins adducin and protein 4.1 are recruited to sites of EPEC pedestals as well as sites of S . Typhimurium and L. monocytogenes invasion. Individual siRNA knockdowns of each spectrin cytoskeletal protein significantly impeded the ability of EPEC to form pedestals and inhibited the abilities of both S .Typhimurium and L.monocytogenes to invade host cells. Further studies identified spectrin cytoskeleton recruitment to later time‐points of S .Typhimurium and L. monocytogenes infections during the intercellular stage of pathogenesis, revealing novel cytoplasmic roles for the spectrin cytoskeleton. This work demonstrates that the spectrin cytoskeletal system is crucial for bacterial pathogenesis and represents a potential target for therapeutic treatment for a broad list of infections. Grant Funding Source : CIHR