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Dab2 in early skeletal muscle development
Author(s) -
SHANG Na,
Samuel Mok C.,
ZHAO Hui,
CHAN Wood Yee
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.874.2
Subject(s) - myogenesis , myf5 , c2c12 , myod , myocyte , skeletal muscle , pax3 , microbiology and biotechnology , biology , xenopus , gene knockdown , myogenic regulatory factors , anatomy , genetics , cell culture , transcription factor , gene
Dab2 is an intracellular adaptor protein and a potential tumor suppressor. In mouse embryos, our study indicated that Dab2 was first expressed in the medial aspect of the dermomyotome at E9.5, and then co‐localized with the early muscle markers Pax3 and Myf5 at the ventrolateral lip of the dermomyotome at E10.5. It has also been known that Dab2 is involved in the MAPK signaling pathway, which is crucial to the muscle development. These observations suggested the potential role of Dab2 in the early skeletal muscle development. To further prove this role, Xenopus laevis embryos and C2C12 myoblasts were employed as in vivo and in vitro models in this study. In situ hybridization results showed that XDab2 was expressed in somites of Xenopus embryos and co‐localized with the muscle markers Pax3 and MyoD. Knockdown of XDab2 expression with antisense morpholinos in somites down regulated the expression of these two muscle markers. When C2C12 myoblasts were induced to differentiate into myotubes, Dab2 expression was simultaneously increased. Suppression of Dab2 expression with miRNAs during myotube formation resulted in reduced myoblast fusion and decreased numbers of myotubes. Our results indicated that Dab2 plays an essential role in the early development of skeletal muscle.