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Phrenic Nerve Morphology in Normotensive and Spontaneously Hypertensive Rats with chronically induced diabetes
Author(s) -
Silva Ferreira Renata,
Sanada Luciana Sayuri,
Salgado Hélio Cesar,
Fazan Valéria Paula Sassoli
Publication year - 2011
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.25.1_supplement.868.11
Subject(s) - medicine , streptozotocin , phrenic nerve , diabetes mellitus , perfusion , intracardiac injection , endocrinology , blood pressure , respiratory system
The aim of this study was to investigate morphometric alterations in phrenic nerves of spontaneously hypertensive rats (SHR), compared to normotensive rats (WR), both with chronically induced diabetes (SHR+STZ and WR+STZ, respectively). WR+STZ and SHR+STZ received a single intravenous injection of streptozotocin (STZ) while WR and SHR received vehicle. Twelve weeks after injection, rats were anesthetized and had their blood pressure and heart rate recorded. After intracardiac perfusion, right and left phrenic nerves were prepared for light microscopy. Morphometric analysis was performed with the aid of computer software, and took into consideration the fascicle area and diameter, as well as myelinated fiber number, density, area and diameter. No differences in fascicular morphology were observed between groups and the phrenic nerves were statistically symmetrical longitudinally and laterally in all groups studied. However, both diabetic groups showed alteration of the myelinated fibers. Despite little alteration on phrenic nerve morphology in SHR+STZ, this study provides a morphological basis for future neuropathy studies in these models of human diseases. Support: CNPQ, CAPES, FAPESP and FAEPA